Cutting the jumper should make zero difference for the regular Arduino Nano, because they’re also connected on the board itself.
I probably didn’t even need to connect them together on the Jumperless.
And the way those solder jumpers work, you can always put a glob of solder to reconnect them.
I’ll try it out now that I have one of those RP2040 connect boards. I don’t think flashing directly from the Wokwi sketch will work because it’s hardcoded to compile the code for a regular ATMega328 based Nano, at some point I’ll add a selection for that.
Not sure about flashing it over one wire via the UART pins, I’ll try it and let you know.